Distinct Molecular Effects of Angiotensin II and Angiotensin III in Rat Astrocytes
Figure 2
Effect of AMA and GluP on Ang II- and Ang III-induced SAPK/JNK protein phosphorylation. Quiescent monolayers of brainstem ((a) and (b)) and cerebellum ((c) and (d)) astrocytes were pretreated with 10 μM AMA or 100 μM GluP for 15 minutes. The cells were subsequently stimulated with 100 nM Ang II or 100 nM Ang III for 10 minutes. Phosphorylated-SAPK/JNK immunoreactive protein levels were measured by Western blot analysis using an antibody specific for the phosphorylated form of SAPK/JNK. Protein loading was quantified using the SAPK/JNK protein antibody. The data were analyzed by densitometry, and the amount of phosphorylation was calculated as the fold increase over basal in the presence of vehicle. Each value represents the mean ± SEM of preparations of brainstem and cerebellum astrocytes from 6 or more litters of neonatal rat pups. *denotes that as compared to basal levels for SAPK/JNK expression in astrocytes prepared from the brainstem and the cerebellum.
