Strategies Aimed at Nox4 Oxidase Inhibition Employing Peptides from Nox4 B-Loop and C-Terminus and p22phox N-Terminus: An Elusive Target
Figure 3
Testing whether N-terminal tail peptides inhibit Nox4 activity. (a) The first 11 amino acids of are displayed. The peptide sequence of between amino acids 6 and 11 is shown (p22 WT 6–11; WAMWAN); point mutations of W6/9R (p22 W6/9R; RAMRAN) are indicated in boldface type. The numbers at the N- and C-terminus of WAMWAN indicate the location of the corresponding residues in the amino acid sequence of . (b) COS-Nox4 cell lysates were preincubated with various concentrations of p22 WT 6–11 for 15 min at 24°C, and H2O2 was measured using Amplex Red. Data represent the mean ± SEM of 3 experiments. (c) COS-Nox4 cell lysates were preincubated with various concentrations of p22 W6/9R for 15 min at 24°C, and H2O2 was measured using Amplex Red. Data represent the mean ± SEM of 3 experiments. indicates a significant difference between COS-22 and COS-Nox4 cell lysates. # indicates a significant difference between COS-Nox4 + 0 μM peptide and COS-Nox4 + DPI.
