Interleukin-2/Anti-Interleukin-2 Immune Complex Expands Regulatory T Cells and Reduces Angiotensin II-Induced Aortic Stiffening
Figure 1
Induction of CD4+CD25+Foxp3+ lymphocyte expansion with the IL-2/ complex. Mice were injected i.p. daily with IL-2/anti-IL-2 JES6-1 mAb (IL-2/) or PBS for 5 days. Ang II was then infused via osmotic Alzet pumps on day 7. Thereafter, mice received IL-2/anti-IL-2 or PBS 3 times weekly throughout the 14 days of Ang II treatment. Mice receiving PBS infusion serve as a baseline control. Lymphocytes isolated from the spleens were then analyzed with flow cytometry. (a) Representative flow cytometry analysis of Tregs, which express CD4, CD25, and Foxp3. (b) The mean percentage of Tregs gated from the CD4+ population shows a significant increase in Tregs after IL-2/ complex treatment. (c) Total splenic CD4+CD25+Foxp3+ cell number. (d) Total splenic CD4+ and CD8+ cell number. per group. Data are means ± SEM.
