p38/JNK Is Required for the Proliferation and Phenotype Changes of Vascular Smooth Muscle Cells Induced by L3MBTL4 in Essential Hypertension

Figure 3

p38/JNK was required for VSMCs proliferation mediated by L3MBTL4. (a) Relative mRNA expression of L3MBTL4 in negative control (NC) and L3MBTL4 overexpression VSMCs. (b) L3MBTL4 protein levels in NC and L3MBTL4 overexpression VSMCs were measured by Western blot. (c) Western blot analysis validated the phosphorylation levels of p38 and c-Jun N-terminal kinase (JNK) in NC and L3MBTL4 overexpression VSMCs. (d) Proliferation of VSMCs in NC, L3MBTL4 overexpression VSMCs, L3MBTL4 overexpression VSMCs + SP600125, and L3MBTL4 overexpression VSMCs + SB2003580 was measured by BrdU incorporation assay. (e) PCNA expression in each group was determined by Western blot (n = 3). ; . All data represent mean ± SEM.

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