miR-145 Alleviates Smooth Muscle Cell Phenotype Transition via ADAM17-Mediated ACE2 Shedding

Figure 2

Expression of miR-145, ADAM17, ACE2, and Mas receptor in the aortic arteries of hypertensive rats. 22 male Wistar rats were selected randomly to receive standard diet or high-sucrose/high-fat diet for 30 weeks, the thoracic aorta was collected, the expression of ACE2 was detected by Western blotting, and the expression of MASR, miR-145, and ADAM17 was detected by qPCR. (a) Quantification of ACE2 expression from panel (b) data was expressed after normalization to the β-actin. (b) Representative figures from Western blotting of ACE2. (c–e) Quantification of mRNA expression of MASR, miR-145, and ADAM17 (qPCR). between groups and between groups.

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