miR-145 Alleviates Smooth Muscle Cell Phenotype Transition via ADAM17-Mediated ACE2 Shedding

Figure 5

ADAM17 siRNA reversed phenotype transition induced by miR-145 in vitro. VSMCs were treated with miR-145 inhibitor in the presence or absence of ADAM17 siRNA (100 nM) as indicated for 48 hours. The expression of α-SMA (a), SM22α (b), OPN (c), EREG (d), and MMP2 (e) protein was detected by Western blotting. All the data were normalized to that of GAPDH. (f) Representative figures of OPN, α-SMA, SM22α, EREG, and MMP2 (Western blotting). (g) The luciferase reporter assay is shown. Cells were transfected with a reporter vector psiCHECK-2-ADAM17 3′-UTR plus either miR-145-5p or the negative control. vs. control group or miR-145-5p NC and ^## vs. miR-145 inhibitor group. All the data are expressed as mean ± SEM of three independent experiments. NC siRNA, negative control siRNA. WT, wide type. MT, mutant type.

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