miR-145 Alleviates Smooth Muscle Cell Phenotype Transition via ADAM17-Mediated ACE2 Shedding

Figure 6

ADAM17 mediated miR-145-induced effect by regulating ACE2-Ang-(1–7)-Mas axis in vitro. VSMCs were treated with miR-145 inhibitor in the presence or absence of ADAM17 siRNA (100 nM) as indicated for 48 hours. (a) Concentration of Ang-(1–7) in the supernatant (ELISA). (b) Representative figures of ACE2 and MASR (Western blotting). Quantification of ACE2 (c) and MASR (d) expression determined by Western blotting, and the data were normalized to that of GAPDH. vs. control group; vs. control group; and ^## vs. miR-145 inhibitor group. All the data are expressed as mean ± SEM of three independent experiments. NC siRNA, negative control siRNA.

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