Activation of T Lymphocytes in Response to Persistent Bacterial Infection: Induction of CD11b and of Toll-Like Receptors on T Cells
Figure 2
Expression of TLR on peripheral CD4+ T cells in patients with implant-associated osteomyelitis and healthy donors: (a) by cytofluorometry, expression of TLR1, TLR2, and TLR4 on CD4+ cells was determined. Each dot represents a patient or a donor, respectively. The horizontal bars represent the mean values. These were different between patients and donors as calculated by ANOVA. (b) When the gate (P2) was set for CD4+ cells, TLR positive cells could be identified (P3) (middle panel) and the expression of CD28 and CD11b on these cells could be determined (right panel). Four cell populations became apparent CD28-CD11b+ (Q1), CD28+CD11b+ (Q2), CD28-CD11b- (Q3), and CD28+CD11b- (Q4). The markers to determine positive or negative were set according to the isotype controls (not shown). The numbers refer to the percentage of cells in the respective quadrant and are calculated as per cent of TLR positive cells. In (b) an example for a single patient is shown, in (c) data of 10 patients and 9 donors are summarised in box and whiskers plots, with the horizontal bar showing the median, and the dot showing the mean values. (Again, markers to defines positive or negative were set according to the appropriate isotype control.)
