Interleukin-1β Expression Is Required for Lysophosphatidic Acid-Induced Lymphangiogenesis in Human Umbilical Vein Endothelial Cells
Figure 3
Lysophosphatidic acid- (LPA-) stimulated vascular endothelial growth factor- (VEGF-) C protein expression is mediated through endothelial growth factor receptor (EGFR) transactivation-, matrix metalloproteinase- (MMP-), LPA1/3-, and interleukin- (IL-) 1R-dependent mechanisms in human umbilical vein endothelial cells (HUVECs). Starved HUVECs were pretreated with AG1478 (100 nM), AF12198 (10 nM), GM6001 (10 M), Ki16425 (10 M), and toxin B (10 nM) for 1 h, followed by LPA (5 M) treatment for an additional 24 h. Conditioned medium was measured by an ELISA as described in “Methods”. All ELISA data are expressed as the mean ± SD from at least three independent experiments. , , and indicate a statistically significant difference with the untreated control.
