MMP-Activated Fluorescence Imaging Detects Early Joint Inflammation in Collagen-Antibody-Induced Arthritis in CC-Chemokine Receptor-2-Null Mice, In-Vivo
Figure 2
In vivo quantification of protease activation in acute arthritis. Comparison of the mean fluorescence intensity in acute inflammation in CAIA mice, in vivo. Hind and fore paw inflammation recruited activated monocytes and neutrophils in Ccr2−/− DBA1/J () mice. Recruited cells were visualized with MMPsense 680, a molecular probe that fluoresces upon specific cleavage by MMP 2, 3, 9, and 13, imaged on IVIS spectrum at excitation of 675 nm and emission of 720 nm. *The right paws and hind feet of the mice increased activity as seen by the values, but the inflammation was not visually apparent. Photons are listed, data presented as mean ± SD.
