Chemokine Expression in Human Astrocytes in Response to Shiga Toxin 2
Figure 5
Expression of IL-8 mRNA in HASTs upon stimulation with Stx2 and IL-1β. Expression of IL-8 mRNA was examined by real-time quantitative PCR using HASTs treated with 10−9 mol/L Stx2 and 10−6 mol/L IL-1β for 24 h. The levels of induction of IL-8 mRNA expression were calculated relative to those of the control cultures, which had not been treated with Stx2 or IL-1β. Data are expressed as fold increases of mRNA expression (mean ± SD from five independent experiments). Comparisons between the levels of IL-8 mRNA elicited by treatment with 10−9 mol/L Stx2 alone and those elicited with 10−9 mol/L Stx2 and 10−6 mol/L IL-1β are shown. Induction of IL-8 mRNA upon stimulation with 10−9 mol/L Stx2 and 10−6 mol/L IL-1β in combination at 24 h was significantly elevated in comparison with that elicited by either 10−9 mol/L Stx2 or 10−6 mol/L IL-1β alone. .
