International Journal of Inflammation / 2012 / Article / Fig 3

Research Article

Rapamycin Augments the NMDA-Mediated TNF Suppression of MRSA-Stimulated RAW264.7 Murine Macrophages

Figure 3

(a) Effects of rapamycin on MRSA RAW264.7 murine macrophages stimulated by Δ-PSM-α LAC. Δ-PSM-α LAC (USA300 PSM-α-depleted strain) stimulated more TNF secretion by RAW264.7 murine macrophages when exposed to rapamycin at 0.1 and 1 ng/mL and less when exposed to rapamycin at 10 and 100 ng/mL. The response at 0.1 ng/mL (first column) had an increase of 7%, not significantly different ( ) than the untreated control (first column). The response at 1 ng/mL (third column) had an increase of 20%, and the responses at 10 ng/mL (fourth column) and 100 ng/mL (fifth column) demonstrated decreases of 17% and 11%, all significantly different from the control ( ). The results are depicted as fold of change compared to the control with 95% confidence intervals shown as “error bars.” . The control response was  pg/mL. (b) Effects of rapamycin on RAW264.7 murine macrophages stimulated by LPS. Lipopolysaccharide (LPS) stimulated more TNF secretion by RAW264.7 murine macrophages when exposed to rapamycin at 0.1 and 1 ng/mL and less when exposed to concentrations of 10 and 100 ng/mL. The response at 0.1 ng/mL had an increase of 9%, significantly different ( ) from the untreated control (first column). The response at 1 ng/mL had an increase of 7% (not significantly different), and the responses at 10 ng/mL (fourth column) and 100 ng/mL (fifth column) demonstrated decreases of 20% and 26%, both significantly different from the control ( ). The results are depicted as fold of change compared to the control with 95% confidence intervals shown as “error bars.” . The control response was 32,486 pg/mL ± 650 pg/mL.
542727.fig.003a
(a)
542727.fig.003b
(b)

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