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International Journal of Inflammation
Volume 2012, Article ID 752015, 6 pages
Research Article

Anti-Inflammatory Activity of Lactobacillus on Carrageenan-Induced Paw Edema in Male Wistar Rats

1Department of Microbiology, Barkatullah University, Bhopal 462026, India
2Department of Biotechnology, Dr. B. R. Ambedkar University, Agra, 282004, India
3Institute of Biomedical Sciences, Bundelkhand University, Jhansi 284001, India
4Department of Zoology, Institute of Basic Sciences, Bundelkhand University, Jhansi, 284001, India

Received 14 September 2011; Revised 1 November 2011; Accepted 12 December 2011

Academic Editor: Takanori Kanai

Copyright © 2012 Sarika Amdekar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Introduction. Lactobacillus casei and Lactobacillus acidophilus were used to assess the anti-inflammatory properties in carrageenan induced acute inflammatory model. Materials and Methods. Diclofenac sodium was used as standard drug at concentration of 150 mg/kg of body weight. Culture of Lactobacillus   2 × 1 0 7  CFU/ml was given orally. Edema was induced with 1% carrageenan to all the groups after one hour of the oral treatments. Paw thickness was checked at 𝑡 = 1 , 2, 3, 4, 5, and 24 hours. Stair climbing score and motility score were assessed at 𝑡 = 2 4 hours. Cytokines assay for IL-6, IL-10, and TNF-α was performed on serum samples. Results. Lactobacillus showed a statistically significant decrease in paw thickness at 𝑃 < 0 . 0 0 1 . L. acidophilus and L. casei decreased by 32% and 28% in paw thickness. They both significantly increased the stair climbing and motility score. Lactobacillus treatment significantly downregulated IL-6 and TNF-α while upregulated IL-10 at 𝑃 < 0 . 0 0 0 1 . Conclusion. L. casei and L. acidophilus significantly decreased the inflammatory reactions induced by carrageenan. This study has also proposed that Lactobacillus ameliorated the inflammatory reaction by downregulating the proinflammatory cytokines pathway.