Resident Corneal Cells Communicate with Neutrophils Leading to the Production of IP-10 during the Primary Inflammatory Response to HSV-1 Infection

Figure 5

Cultured fibroblast cells produce IP-10 in response to stimulation with proinflammatory mediators. 4 × 10⁴ cultured mouse corneal fibroblast cells were incubated at 37°C and 5% CO₂ in 500 μL serum-free media with or without cytokine stimulation for 24 h. Cells were stimulated with 10 ng/mL of either recombinant mouse IL-α or IFN-γ and assayed for IP-10 production by ELISA.