Resident Corneal Cells Communicate with Neutrophils Leading to the Production of IP-10 during the Primary Inflammatory Response to HSV-1 Infection
Figure 7
Neutrophil and NK depletion lead to a significant reduction of IP-10 in the HSV-1-infected cornea. Different groups of mice () were depleted of each subset of cells by intraperitoneal injection 1 mg mAb RB6 8C5 (neutrophils), 1 mg antiasialo GM1 antibody admixed with 0.1 mg mAb, NK1.1 (NK cells) or 0.5 mg GK1.5 antibody (CD4+ cells) three hours prior to intrastromal injection of PFU HSV-1. Control animals received intraperitoneal injection of rat IgG prior to virus infection at the same dose. At days 2 and 3 post infection corneas were collected and processed for chemokine analysis via ELISA. Levels of IP-10 protein production within the infected cornea are shown as pg/mL for each sample. Cellular depletions were confirmed as described in the materials and methods. N.D. = not done.
