Prostaglandin E₂ Does Not Modulate CCR7 Expression and Functionality after Differentiation of Blood Monocytes into Macrophages

Figure 3

Differentiation of Mono Mac-1 cells into MDMM-1 cells induces the loss of CCR7 expression. Mono Mac-1 cells were differentiated in MDMM-1 cells as described. (a) CCR7 surface expression was evaluated by flow cytometry on Mono Mac-1 and MDMM-1 cells following treatment with 1 μM PGE₂ for 24 h. Data are the mean of four representative experiments, and representative histograms obtained for Mono Mac-1 (b) and (c) MDMM-1 cells are presented. ^∗𝑃<0.5, ^∗∗𝑃<0.005, ^∗∗∗𝑃<0.001, and ^∗∗∗∗𝑃<0.0001.

(a)

(b)

(c)