Limited Applicability of GW9662 to Elucidate PPARγ-Mediated Fatty Acid Effects in Primary Human T-Helper Cells
Figure 3
GW9662 dose-dependently causes cell death in leukocytes. PBMC were incubated for a total of 24 h with increasing concentrations of GW9662. After 19 h, stimulants were added for further 5 h. Cell viability was flow cytometrically assessed by annexin-V and propidium iodide exclusion double staining and is expressed as % of control without GW9662 (dotted line). Annexin-V positive and PI negative cells were defined as early apoptotic cells; annexin-V positive and PI positive cells were defined as late apoptotic and necrotic cells. (a) Data are expressed as means ± SEM of . The dose-dependent effect is statistically significant with . (b) Representative dot plots of GW9662 treated PBMC, gated for lymphocytes.
