Interleukin-17A Exacerbates Ferric Chloride-Induced Arterial Thrombosis in Rat Carotid Artery

Figure 1

Effect of IL-17A on thrombus mass induced by FeCl₃ application on rat carotid artery. A filter paper soaked in FeCl₃ (5%) or in recombinant mouse IL-17A (100 μg/mL) was applied onto the external surface of the right carotid artery, for 30 minutes; afterward the paper was removed and the vessel was left in situ for 60 minutes, to enable thrombus formation. In another set of experiments, an IL-17A (100 μg/mL), or vehicle (HCl, 4 mM), soaked filter paper was applied onto the vessel for 30 minutes before applying FeCl₃ (5%). At the end of 60-minute period, a piece of about 2 cm in length of the right carotid artery and of its contralateral was excised and weighed. Thrombus size was evaluated by the difference in weight between the treated vessel and its contralateral. All controls were performed by applying only vehicle (H₂O or HCl 4 mM). ^##𝑃<0.01 versus FeCl₃, ***𝑃<0.001 versus IL-17A, and °𝑃<0.05 versus vehicle (one way ANOVA followed by Bonferroni’s test; 𝑛=8–10).