Posttranscriptional Suppression of Lipopolysaccharide-Stimulated Inflammatory Responses by Macrophages in Middle-Aged Mice: A Possible Role for Eukaryotic Initiation Factor 2α

Figure 1

LPS-stimulated production of TNF-α by peritoneal macrophages from young and middle-aged mice. Resident peritoneal macrophages (pooled cells from four mice per each age group) from young or middle-aged mice were cultured with or without 100 ng/mL LPS for 6 h. The experiment was independently performed three times. (a) The concentrations of TNF-α in the culture supernatants were measured by ELISA. Values: means ± SEM (𝑛=3). ^∗𝑃<0.05. (b) Total RNA extracted from macrophages was converted to cDNA to determine the levels of TNF-α mRNA by PCR. The values are corrected with the levels of GAPDH mRNA and 18S rRNA. Values: means ± SEM (𝑛=3). ^∗∗𝑃<0.01.

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