Research Article

Posttranscriptional Suppression of Lipopolysaccharide-Stimulated Inflammatory Responses by Macrophages in Middle-Aged Mice: A Possible Role for Eukaryotic Initiation Factor 2α

Figure 2

LPS-stimulated JNK phosphorylation of peritoneal macrophages from young and middle-aged mice. Resident peritoneal macrophages (pooled cells from four mice per each age group) from young or middle-aged mice were cultured with or without 100 ng/mL LPS for 6 h. (a) Phosphorylation of the p54 subunit of JNK, (b) abundances of the p54 subunit of JNK, and (c) phosphorylation of the p46 subunit of JNK were analyzed by Western blotting. Values are shown as means from duplicate samples.
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