Posttranscriptional Suppression of Lipopolysaccharide-Stimulated Inflammatory Responses by Macrophages in Middle-Aged Mice: A Possible Role for Eukaryotic Initiation Factor 2α
Figure 3
LPS-stimulated NF-κB phosphorylation of peritoneal macrophages from young and middle-aged mice. Resident peritoneal macrophages (pooled cells from four mice per each age group) from young or middle-aged mice were cultured with or without 100 ng/mL LPS for 6 h. (a) Phosphorylation of the p65 subunit of NF-κB, (b) phosphorylation of IκBα, and (c) abundances of IκBα were analyzed by Western blotting. Values are shown as means from duplicate samples.
