Research Article

Posttranscriptional Suppression of Lipopolysaccharide-Stimulated Inflammatory Responses by Macrophages in Middle-Aged Mice: A Possible Role for Eukaryotic Initiation Factor 2α

Figure 4

Phosphorylation of eIF-2α and PKR in peritoneal macrophages from young and middle-aged mice. Resident peritoneal macrophages (pooled cells from four mice per each age group) from young or middle-aged mice were cultured with or without 100 ng/mL LPS for 6 h. (a) Phosphorylation of eIF-2α, (b) abundances of PKR, and (c) phosphorylation of PKR were analyzed by Western blotting. Values are shown as means from duplicate samples.
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