Posttranscriptional Suppression of Lipopolysaccharide-Stimulated Inflammatory Responses by Macrophages in Middle-Aged Mice: A Possible Role for Eukaryotic Initiation Factor 2α
Figure 5
Expressions of ATF4 mRNA and PERK protein in peritoneal macrophages from young and middle-aged mice. Resident peritoneal macrophages (pooled cells from four mice per each age group) from young or middle-aged mice were cultured with or without 100 ng/mL LPS for 6 h. (a) Total RNA extracted from macrophages was converted to cDNA to determine the levels of ATF4 mRNA by PCR. The values are corrected with the levels of GAPDH mRNA and 18S rRNA. Values: means ± SEM (). (b) Abundances of PERK were analyzed by Western blotting.
