Posttranscriptional Suppression of Lipopolysaccharide-Stimulated Inflammatory Responses by Macrophages in Middle-Aged Mice: A Possible Role for Eukaryotic Initiation Factor 2α
Figure 7
Effects of salubrinal on the LPS-stimulated inflammatory responses of RAW264.7 cells. RAW264.7 cells were stimulated with 100 ng/mL LPS in the presence of 10 μM salubrinal or DMSO alone for 6 h. (a) Concentrations of TNF-α in the culture supernatants, measured by ELISA. Values: means ± SEM (). . (b) Total RNA from macrophages was converted to cDNA to determine the levels of TNF-α mRNA by PCR. Values: means ± SEM (). (c) Phosphorylation of the p54 subunit of JNK and (d) abundances of the p54 subunit of JNK were analyzed by Western blotting. Values are shown as means from duplicate samples.
