Posttranscriptional Suppression of Lipopolysaccharide-Stimulated Inflammatory Responses by Macrophages in Middle-Aged Mice: A Possible Role for Eukaryotic Initiation Factor 2α

Table 1

Primers used in the RT-PCR.

Gene	Forward primer sequence	Reverse primer sequence	Product size (bp)	Cycle number
TNF-α	5′-GGA GGG AGA ACA GAA ACT CCA GAA-3′	5′-ATG AGA GGG AGG CCA TTT GGG AA-3′	339	27
ATF4	5′-ATG AGC TTC CTG AAC AGC GAA GTG-3′	5′-GAA CAG GGG AAA GGC TGC AAG AAT-3′	485	30
GAPDH	5′-CAG TAT GAC TCC ACT CAC GGC AAA-3′	5′-CAG TGA TGG CAT GGA CTG TGG T-3′	406	22
18S rRNA	5′-TGG TGC ATG GCC GTT CTT AGT T-3′	5′-GCA AGC TTA TGA CCC GCA CTT ACT-3′	346	22