Macrophage Derived Platelet Activating Factor Implicated in the Resolution Phase of Gouty Inflammation
Figure 2
Platelet activating factor (PAF) production by D7 in vitro differentiated macrophages incubated with MSU crystals and with or without WEB2086 (WEB). Monocytes were cultured for 1 and 7 days and stimulated with MSU crystals and collected supernatants were analysed for PAF by ELISA. Results are mean and SEM of 4 experiments. D7 = supernatants from day 7 macrophages, D1 = supernatants from day 1 Monocytes, and WEB = WEB2086. Macrophages also secreted PAF when incubated with LPS ( mean ± SEM; ng/mL per 106 cells), data not shown.
