Carbon Black Particle Exhibits Size Dependent Toxicity in Human Monocytes

Figure 6

Proinflammatory cytokine and chemokine level in THP-1 cells exposed to carbon particles at their TC-50 concentration (nano carbon: 591.4 μg/mL and micro carbon 687.1 μg/mL) for different times (0, 6, 18, 24, and 48 h) as determined by ELISA. A positive control for each assay was run in parallel as per manufacturer’s instruction. (a) IL-1β level, (b) IL-6 level, (c) TNF-α level, and (d) IL-8 level. Results are presented as mean ± SEM of three independent replicate experiments. Significance is indicated by ^∗∗∗𝑃<0.001 versus control. ^#𝑃<0.001 indicates significant difference between the particle sizes.

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