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International Journal of Inflammation
Volume 2015, Article ID 241738, 9 pages
Clinical Study

Cytokines Modulate the “Immune-Metabolism” Interactions during Behçet Disease: Effect on Arginine Metabolism

1USTHB, “Cytokines and NO Synthases” Team, LBCM, FSB, BP 32 El Alia, Bab Ezzouar, 16111 Algiers, Algeria
2Internal Medicine Department, CHU Mustapha Bacha, Algiers, Algeria
3Internal Medicine Department, CHU Bab El Oued, Algiers, Algeria
4Ophthalmology Department, CHU Ibn Rochd, Annaba, Algeria
5EA 2216, UBO, Brest, France

Received 13 June 2014; Revised 15 September 2014; Accepted 4 November 2014

Academic Editor: John Ryan

Copyright © 2015 Houda Belguendouz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Aim and Methods. In this study, we evaluated NOS and arginase activities and their regulation during Behçet disease, a systemic chronic inflammatory disorder with uncertain etiology. The peripheral blood mononuclear cells of 36 patients and 15 control samples (PBMC) were cultured in either RPMI 1640, MEM, or DMEM complemented with 10% of FBS and antibiotics. Cultures were performed with or without the control or patients plasma. Subsequent treatment contained anticytokines (IL-6, TGF-β), a mitogenic effector (PHA), or NOS modulators (L-NMMA, BH4). Culture supernatants were harvested after 24 h of incubation. NO and urea measurements were, respectively, performed by modified Griess and Berthelot methods. Results. Higher urea levels were found in patients’ plasma compared to the control’s (P < 0.05). NOS modulators induced inverted production profiles for NO and urea (P < 0.05). Their results differed depending on the clinical findings (P < 0.05). It was also found that cytokine neutralization induced different response profiles in patients as opposed to control cultures (P < 0.05). Conclusion. Our results suggest that arginases can compete with NOS2 for L-arginine during Behçet disease. Both enzymes are regulated by environmental cytokines and substrate availability. Furthermore, it seems that NOS/arginase balance is dependent on clinical expression.