EOLA1 Inhibits Lipopolysaccharide-Induced Vascular Cell Adhesion Molecule-1 Expression by Association with MT2A in ECV304 Cells

Figure 1

Subcellular location of EOLA1 is indicated in ECV304 cells. ECV304 cells were transfected with the blank plasmid pEGFP-N2 and the fusion protein expression plasmid pEGFP-EOLA1. After 48 h culture, the transfected cells were fixed with 4% PFA. (a) The images of ECV304 cells were taken under the optical microscope. (b) The cells were incubated with rat anti-GFP antibody (primary antibody) and goat anti-rat antibody (secondary antibody), stained with DAPI, and photographed under LSM510 META laser confocal microscope. (c) The cells were incubated with rabbit anti-EOLA1 polyclonal antibody (primary antibody) and HRP-labeled goat anti-rabbit IgG antibody (secondary antibody), stained with HRP-labeled streptoantibiotin and diaminobenzidine, and embedded with epoxy resin 618. Finally the cells were made into ultrathin sections, stained with lead, and observed and photographed under TECNA110 transmission electron microscope. The white arrow identifies immune sediment deposition in ECV304 cells transfected with pEGFP-EOLA1 by anti-EOLA1 antibody.

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