EOLA1 Inhibits Lipopolysaccharide-Induced Vascular Cell Adhesion Molecule-1 Expression by Association with MT2A in ECV304 Cells

Figure 5

EOLA1 mediates LPS-induced VCAM-1 production by MT2A. (a) ECV304 cells were transfected with MT2A shRNA or control shRNA, and the cells were lysed and analyzed by Western blotting with anti-MT2A or ant-EOLA1 antibody, β-actin as a loading control. (b) ECV304 was transfected with MT2A shRNA or control shRNA and then stimulated with LPS (100 ng/mL) for 6 h. VCAM-1 production was assayed by ELISA. Data represent the mean ± SD of three independent experiments ().

(a)

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