Ethanol-Induced Alterations of T Cells and Cytokines after Surgery in a Murine Infection Model

Figure 2

Analysis of body weight and clinical score in a murine model of ethanol exposition for 2 weeks, surgery, and pulmonary infection. (a) Development of body weight in sham-infected animals (left panel) and in animals with pulmonary infection with Klebsiella pneumoniae (right panel) over course of time in ethanol-treated (black/white triangle) and respective saline-treated controls (black circles/white circles). when compared to time-matched control group; data are presented as mean ± SEM, Mann–Whitney U test ( each group). (b) Development of clinical score in sham-infected animals (left panel) and in animals with pulmonary infection with Klebsiella pneumoniae (right panel) over course of time in ethanol-treated (black/white triangle) and respective saline-treated controls (black circles/white circles). For clinical scoring, motor activity, mucous membranes, and piloerection were assessed. For each item a score from zero to four points was given (0 points = no clinical impairment, 1 point = mild, 2 points = moderate, 3 points = moderate to severe, and 4 points = very severe impairment of the respective item). Clinical rating score was evaluated for each group. when compared to time-matched control group; data are presented as mean ± SEM, Mann–Whitney U test ( each group). ETOH: ethanol treatment; NaCl: saline-treated control; K. pneumoniae: pulmonary infection with Klebsiella pneumoniae.

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