Identification of a Novel Alternatively Spliced Form of Inflammatory Regulator SWAP-70-Like Adapter of T Cells

Figure 2

Kinetics of SLAT2 expression in differentiated Th cells. (a) CD4⁺ T cells from B6 mice were differentiated under Th1- and Th2-inducing conditions as in Figure 1. Cell lysates were prepared at the indicated days and analyzed by Western blotting using anti-SLAT Ab. Numbers under the SLAT blot indicate intensity of SLAT and SLAT2 signals relative to SLAT signal of Th2 cells on day 0 (=1) after normalization to the actin signal. (b) CD4⁺ T cells from OT-II mice were differentiated under Th1- or Th2-inducing conditions with splenic APCs and 1 μg/mL peptide for 5 days, and the cells were further restimulated with plate-bound anti-CD3 plus -CD28 mAbs. Cell lysates were prepared at the indicated days and analyzed as in (a). (c) CD4⁺ T cells from B6 mice were differentiated into the indicated Th cell subset and restimulated with splenic APCs and anti-CD3 Ab for 4 days. Cell lysates were analyzed by Western blotting as in (a). Data are representative of at least two independent experiments.

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