Inhibition of Proinflammatory Cytokines in Cutibacterium acnes-Induced Inflammation in HaCaT Cells by Using Buddleja davidii Aqueous Extract

Figure 1

B. davidii aqueous extracts inhibited the expression of TLR-2 in C. acnes-treated HaCaT cells. The expression level of TLR-2 mRNA was determined using a RT-PCR. HaCaT cells were seeded in six-well plates for 24 hours and then were pretreated with various concentrations (12.5, 25, 50, and 100 µg/ml) of B. davidii aqueous extracts for 1 hour and incubated with heat-killed C. acnes (100 MOI) for 24 hours. The values are expressed as the mean ± SD of three independent experiments, vs C. acnes-free and without B. davidii aqueous extract, , vs C. acnes and without plants aqueous extract.