Inhibition of Proinflammatory Cytokines in Cutibacterium acnes-Induced Inflammation in HaCaT Cells by Using Buddleja davidii Aqueous Extract

Figure 2

Reduction of the protein ratio of NF-κB in C. acnes-treated HaCaT cells by B. davidii aqueous extracts. HaCaT cells were pretreated with the indicated concentration of B. davidii aqueous extracts for 1 hour before treatment with heat-killed C. acnes (100 MOI) for 24 hours. Western blot analysis was carried out to evaluate the level of phosphorylated NF-κB or NF-κB. (a) B. davidii aqueous extracts reduced the phosphorylation of NF-κB and C. acnes-induced inflammation. (b) Relative protein expression of p-NF-κB and NF-κB (relative to GAPDH) was determined using densitometry. Control was C. acnes-free without B. davidii aqueous extract. All data were expressed as mean ± SD. compared with C. acnes treated cells only.

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