Inhibition of Proinflammatory Cytokines in Cutibacterium acnes-Induced Inflammation in HaCaT Cells by Using Buddleja davidii Aqueous Extract

Figure 3

Inhibition of B. davidii aqueous extract on MAPKs expression in C. acnes-treated HaCaT cells. Western blot analysis was used to check the amount of protein of p-p38, p38, p-JNK, and JNK. HaCaT cells were pretreated with the indicated concentration of B. davidii aqueous extracts for 1 hour before treatment with heat-killed C. acnes (100 MOI) for 24 hours. (a) Total protein expression and phosphorylation of p38 and JNK were reduced by treatment with B. davidii aqueous extracts. (b) Relative protein expression of p-p38, p38, p-JNK, and JNK (relative to GAPDH) was determined using ImageJ. All data were expressed as mean ± SD. , compared with C. acnes-treated cells only.

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