Inhibition of Proinflammatory Cytokines in Cutibacterium acnes-Induced Inflammation in HaCaT Cells by Using Buddleja davidii Aqueous Extract

Figure 4

B. davidii aqueous extract reduced the level of the proinflammatory cytokines expression in C. acnes-treated HaCaT cells. HaCaT cells were pretreated with B. davidii aqueous extract (12.5, 25, 50, and 100 μg/ml) 1 hour before incubation with C. acnes (100 MOI) for 24 hours. The expression of TNF-α, IL-1β, IL-6, and IL-8 mRNA was then measured using RT-PCR. Experiments were performed in triplicate using independent samples, and the data are presented as the mean ± SD, vs C. acnes-free and without B. davidii aqueous extract, , , vs C. acnes and without plant aqueous extract.

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