The Effect of Lithium on Inflammation-Associated Genes in Lipopolysaccharide-Activated Raw 264.7 Macrophages
The effects of lithium on viability, morphology, and proliferation of Raw 264.7 macrophage cells. To examine the effects of lithium on proliferation and viability of Raw 264.7 macrophages, cells were seeded in 96-well plates at 6 × 106 cells/well and treated with LiCl (0.3125, 0.625, 1.25, 2.5, 5, 10, 2, 0, 50, and 100 mM), NaCl (10 mM), and actinomycin-D (0.02 mg/ml) for 24 hrs. Cells were viewed and captured with the Nikon Ti-E inverted microscope (b); thereafter 5 mg/ml MTT was added for 4 hrs and then absorbance was read using by GloMax-Multi microplate reader at 570 nm (Promega, USA) (a). Graphs were executed using GraphPad Prism 4 software and the statistical analysis was carried out using GraphPad InStat™ software using ANOVA (Tukey–Kramer, ;).
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