International Journal of Inflammation / 2020 / Article / Fig 2

Research Article

The Effect of Lithium on Inflammation-Associated Genes in Lipopolysaccharide-Activated Raw 264.7 Macrophages

Figure 2

The effect of lithium on proliferation and cellular integrity of Raw 264.7 macrophages in real time. Cells were seeded at 40,000 cell/well in an E plate for 24 hrs and then treated with 0.02 mg/ml actinomycin-D, 10 mM NaCl, and various concentrations of lithium (1.25–20 mM (a) and 50–100 mM (b)) for 56 hrs. The xCELLigence (RTCA) software was set to take readings after every 15-minute interval giving a real-time measure of cell size, adhesion, growth, viability, and proliferation.

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