The Effect of Lithium on Inflammation-Associated Genes in Lipopolysaccharide-Activated Raw 264.7 Macrophages

Figure 4

Determination of apoptotic properties shown by lithium on Raw 264.7 macrophage cells using flow cytometry. Apoptosis investigation was carried out by seeding cells at a concentration of 6 × 10⁶ cells/ml overnight in a 200 mm dish. This was then followed by treating the cells with 5, 10, 20, and 50 mM lithium, 10 mM NaCl, and 0.01 and 0.02 mg/ml actinomycin-D for 24 hrs. Cells were thereafter stained with mixture of binding buffer, Annexin-V FITC, and PI for 20 min in the dark, and then cell analysis was carried out using flow cytometer (BD Biosciences, USA) according to the manufacturer’s protocol. (a) Untreated control. (b) LiCl 10 mM. (c) NaCl 10 mM. (d) Actinomycin-D 0.02 mg/ml.

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