The Effect of Lithium on Inflammation-Associated Genes in Lipopolysaccharide-Activated Raw 264.7 Macrophages
Effect of lithium on oxidative burst activated by LPS on Raw 264.7 macrophage cells. Cells were seeded at a density of 6 × 105 cells/ml (200 μl) on a coverslip and pretreated with 10 mM lithium and treated with 10 μg/ml LPS for 24 hrs. The fluorescence measurement was accomplished with inverted fluorescent microscope Nikon Ti-E at 10x magnification (a). Fluorescence intensity of the pictures was measured using the NIS Element view imaging software at 10x magnification (Nikon, Japan). Graphs were executed using GraphPad Prism 4 software and the statistical analysis was carried with GraphPad InStat software using ANOVA (Tukey–Kramer) (;).
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