International Journal of Inflammation / 2020 / Article / Fig 7

Research Article

The Effect of Lithium on Inflammation-Associated Genes in Lipopolysaccharide-Activated Raw 264.7 Macrophages

Figure 7

Determination of effect of lithium on nitric oxide production on LPS-induced Raw 264.7 cells. Cells were seeded at 6 × 106 cell/ml and treated with lithium (5 and 10 mM), 10 mM NaCl, and 100 ng/ml LPS, combining each of the chemicals with LPS for 24 hrs. Cells were stained with DAF2-DA and Hoechst for 20 min at RT. The measurement of the fluorescence was accomplished by using fluorescent inverted Nikon Ti-E microscope at 20x magnification (b). Fluorescence intensity of the pictures was measured using the NIS Element view imaging software (Nikon, Japan) (a). Graphs were executed using GraphPad Prism 4 software and the statistical analysis was carried out using GraphPad InStat software and ANOVA (Tukey–Kramer) (; ).
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