The Resistance to Freeze-Drying and to Storage Was Determined as the Cellular Ability to Recover Its Survival Rate and Acidification Activity

Table 1

Composition and modifications of membrane fatty acids (% of peaks areas) induced by growth temperature of L. paracasei ssp. LMG 9192^𝑇 and L. plantarum CWBI-B1419. The peaks were identified as hexadecanoic (palmitic) acid (C_16:0), hexadecenoic (palmitoleic) acid (C_16:1), octadecanoic (stearic) acid (C_18:0), cis-9-octadecenoic (oleic) acid (C_18:1), cis-7,12-octadecadienoic (linoleic) acid (C_18:2) and cis-9,12,15-octadecatrienoic (linolenic) acid (C_18:3). The hexadecanoic (palmitic) acid (C_16:0) fatty acids represented almost 50% of the total fatty acids.

Fatty acids	Strains (Percentages of peaks (±7%))
	LMG 9192^T			CWBI-B1419
	Control	Sorbitol	Monosodium glutamate	Control	Sorbitol	Monosodium glutamate
C16:0	53.39 ± 0.5	52.90 ± 0.5	53.30 ± 0.4	54.16 ± 0.2	51.60 ± 0.5	52.5 ± 0.3
C16:1	7.79 ± 0.1	7.80 ± 0.2	7.20 ± 0.2	9.50 ± 0.1	7.20 ± 0.3	8.74 ± 0.4
C18:0	9.80 ± 0.2	9.80 ± 0.4	9.40 ± 0.7	9.58 ± 0.4	8.60 ± 0.2	9.21 ± 0.6
C18:1	27.70 ± 0.4	24.9 ± 0.3	26.90 ± 0.1	24.33 ± 0.3	26.40 ± 0.3	26.19 ± 0.7
C18:2	0.40 ± 0.02	0.80 ± 0.07	0.70 ± 0.03	0.42 ± 0.05	0.80 ± 0.04	0.76 ± 0.02
C18:3	0.90 ± 0.01	5.80 ± 0.2	2.50 ± 0.1	2.01 ± 0.2	5.40 ± 0.6	2.60 ± 0.1
Σ	100	100	100	100	100	100
Σ SFA%	63.20 ± 0.3	62.70 ± 0.7	62.70 ± 0.3	63.74 ± 0.1	60.2 ± 0.5	61.71 ± 0.5
Σ UFA%	36.80 ± 0.5	39.30 ± 0.3	37.30 ± 0.4	36.26 ± 0.2	39.80 ± 0.6	38.29 ± 0.3
U/S	0.58 ± 0.02	0.62 ± 0.02	0.59 ± 0.03	0.57 ± 0.01	0.66 ± 0.03	0.62 ± 0.01

Cells were cultivated by batch fermentation at increased temperatures from 37°C and harvested by centrifugation. After freezing for 24 hours, cells were freeze-dried without cryoprotectant and fatty acids were extracted and analysed by CPG. SFA: Saturated fatty acids; UFA: unsaturated fatty acids;. Data were presented as the average of two independent trials with SD. Note that experiment was not a quantitative method.