Flotation—A New Method to Circumvent PCR Inhibitors in the Diagnosis of Lawsonia intracellularis

Table 3

The results from PCR analysis of 116 clinical samples previously judged as positive, negative, or inhibited by boiling and nested PCR, prepared by the finally selected flotation setup. The results from two other, previously used methods are included for comparison.

Sample preparation method		Results
	Positive	Inhibited	Negative
Boiled lysate + nested PCR	24	17	75
QIAamp DNA Stool Mini Kit + PCR	13	1	102
Flotation + PCR	15	2	99