International Journal of Microbiology / 2009 / Article / Tab 2

Research Article

Phenotypic and Genotypic Antimicrobial Resistance Profiles of Campylobacter jejuni Isolated from Cattle, Sheep, and Free-Range Poultry Faeces

Table 2

Primers and probes sequences.

TargetNameSequences ( 5 3 )C (nM)

gyrAgyr-FwGGGTGCTGTTATAGGTCGTTATCA900
gyr-RvTTGAGCCATTCTAACCAAAGCAT900
Probe-gyr-SHEX – CAT[+G]GAGAT[+A][+C][+A]GC[+A]GTTT – BHQ1150
Probe-gyr-RFAM – CATGGAGATATAGCAGTTT – MGB150

23S23S-FwCAGTGAAATTGTAGTGGAGGTGAAA900
23S-RvTTCTTATCCAAATAGCAGTGTCAAGCT900
Probe-23S-SHEX – CGGGGTC[+T][+T][+T]CCGTCTTG – BHQ1100
Probe-23S-RFAM – CGGGGTC[+T][+C][+T]CCGTCTTG – BHQ1200

LNA nucleotides are indicated by a + symbol and in brackets; SNPs are underlined

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