Purification of an Exopolygalacturonase from Penicillium viridicatum RFC3 Produced in Submerged Fermentation

Figure 2

Gel filtration chromatography of PG on Sephadex G-75 (2.5 × 90 cm) equilibrated with 40 mM acetate buffer, pH 4. (a) Crude extract concentrated by ultrafiltration with 10 kDa cut-off, (b) crude extract after ultrafiltration with 10 and 50 kDa cut-off.

(a)

(b)