Purification of an Exopolygalacturonase from Penicillium viridicatum RFC3 Produced in Submerged Fermentation

Figure 3

Ion-exchange chromatography of PG on Q Sepharose (1 × 30 cm) equilibrated with 40 mM acetate buffer, at pH 4.0 and eluted with NaCl (0–1 M).