Purification of an Exopolygalacturonase from Penicillium viridicatum RFC3 Produced in Submerged Fermentation

Table 1

Summary of purification of exo-PG III from Penicillium viridicatum RFC3.

Step	Volume	PG	Protein	U total	S.A.∗	Yield	Fold
	(mL)	(U mL^−1)	(mg mL^−1)		(U mg^−1)	(%)	purification
Crude enzyme	1300	3.8	0.08	4966	49.0	100	—
Ultrafiltration	45	63.9	0.72	2875.5.4	88.8	57.9	9.6
in 10 and 50
kDa cut-off
membranes
Fraction I from	198	5.1	0.03	1003.9	195.0	20.2	21.2
Sephadex G75
column
Fraction I from	35	4.9	0.014	169.8	346.4	3.4	37.7
Q Sepharose
column.

∗S.A. (Specific Activity).