International Journal of Microbiology

Research Article

Comprehensive and Rapid Real-Time PCR Analysis of 21 Foodborne Outbreaks

Table 1

Bacterial strains assayed by SYBR Green I PCR
Bacterial strainsSource s e PCR results with each primer set (see Table 2)
eaeJMS1JMS2LTSTaEAST-1aggR-ZvirAStyinvayadA-XPAGPSGABCC ceuEhlyAtdhtrhAHH1FemBces-TMSGGAP
Escherichia coli -EPECO55(eaeA)EC-273 6 b +−−−−−−−−−−−−−−−−−−−−−
E. coli -EPEC O153 (eaeA and astA)EC-264 9 b +−−−−+−−−−−−−−−−−−−−−−
E. coli -EHEC O26:H11(Stx1)SE-02005++−−−−−−−−−−−−−−−−−−−−
E. coli -EHEC O157:H7 (Stx2)SE020025+−+−−−−−−−−−−−−−−−−−−−
E. coli -EHEC O157:H7 (Stx1 and Stx2)SE-02027+++−−−−−−−−−−−−−−−−−−−
E. coli -ETEC O148 (LT, ST and astA)EC-351 5 b −−−+++−−−−−−−−−−−−−−−−
E. coli -ETEC O169 (ST and astA)EC-472 5 b −−−−++−−−−−−−−−−−−−−−−
E. coli- EAEC O111 (aggR and astA)EC-413 1 b −−−−−++−−−−−−−−−−−−−−−
E. coli -EIEC O124:HNM (virA)EA3 2 a −−−−−−−+−−−−−−−−−−−−−−
Shigella sonneiI00031−−−−−−−+−−−−−−−−−−−−−−
Salmonella EnteritidisSal-2339−−−−−−−−+−−−−−−−−−−−−−
Yersinia enterocolitica O3/B4Pa241−−−−−−−−−+−−−−−−−−−−−−
Y. pseudotuberculosis O4bSP988−−−−−−−−−+−−−−−−−−−−−−
Providencia alcalifaciensNIID12 4 C −−−−−−−−−−+−−−−−−−−−−−
Plesiomonas shigelloidesNIID12 3 C −−−−−−−−−−−+−−−−−−−−−−
Campylobacter jejuniSC 009−−−−−−−−−−−−+−−−−−−−−−
Campylobacter coliSC 011−−−−−−−−−−−−−+−−−−−−−−
Vibrio cholerae O1ATCC14035−−−−−−−−−−−−−−+−−−−−−−
V. cholerae O139NIID63-9 3 C −−−−−−−−−−−−−−+−−−−−−−
V. cholerae non- O1SVP84−−−−−−−−−−−−−−+−−−−−−−
V. parahaemoliticus O3:K6 (tdh)SVP02−−−−−−−−−−−−−−−+−−−−−−
V. parahaemoliticus O3:K6 (trh)NIIDK 4 C −−−−−−−−−−−−−−−−+−−−−−
Aeromonas hydrophila O1ATCC7966−−−−−−−−−−−−−−−−−+−−−−
Staphylococcus aureusSS 0 5 e −−−−−−−−−−−−−−−−−−+−−−
Emetic Bacillus cereusNo.12 7 e −−−−−−−−−−−−−−−−−−−++−
Enterotoxigenic B. cereusNo. 1 e −−−−−−−−−−−−−−−−−−−−+−
Clostridium perfringensH 2 d −−−−−−−−−−−−−−−−−−−−−+
Strain kindly donated by K. Sugiyam a a , Shizuoka Prefectural Institute of Public Health, Shizuoka; J. Yatsuyanag i b , Akita Prefectural Institute of Public Health, Akita; M. Tamura and E. Arakaw a c , f O ther strains except for ATCC numbers are our own collections.