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International Journal of Microbiology
Volume 2010, Article ID 317512, 4 pages
http://dx.doi.org/10.1155/2010/317512
Research Article

New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA

1Infectious Diseases Service, Department of Internal Medicine, University Hospital Center and University of Lausanne, 1011 Lausanne, Switzerland
2Institute of Microbiology, University Hospital Center and University of Lausanne, 1011 Lausanne, Switzerland
3Laboratory of Microbiology, University Hospital of Geneva, Geneva, Switzerland

Received 3 March 2010; Accepted 16 June 2010

Academic Editor: Paula J. Fedorka-Cray

Copyright © 2010 Andres Pascual et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mycoplasma hominis is a fastidious micro-organism causing genital and extragenital infections. We developed a specific real-time PCR that exhibits high sensitivity and low intrarun and interrun variabilities. When applied to clinical samples, this quantitative PCR allowed to confirm the role of M. hominis in three patients with severe extragenital infections.