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International Journal of Microbiology
Volume 2010, Article ID 351397, 5 pages
http://dx.doi.org/10.1155/2010/351397
Research Article

Spa Diversity among MRSA and MSSA Strains of Staphylococcus aureus in North of Iran

1Islamic Azad University of Lahijan, Lahijan, Iran
2Basic Sciences Faculty, Golestan University, Gorgan, Iran
3Golestan University of Medical Sciences, Gorgon 4934174515, Iran
4Microbiology Department, Infectious Disease Research Center, Gorgan, Iran

Received 27 April 2010; Revised 15 June 2010; Accepted 6 July 2010

Academic Editor: Joseph Falkinham

Copyright © 2010 Fatemeh Shakeri et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Protein A of Staphylococcus aureus is a pathogenic factor whose encoding gene, spa, shows a variation in length in different strains. In this study the spa gene variation in S. aureus isolated from healthy carriers and patients was studied, We also compared this variation among MRSA with MSSA strains. 208 strains of Staphylococcus aureus which we were isolated from Gorgan, north of Iran were studied, 121 cases from patients and 87 cases from healthy carriers, 59 out of them were MRSA and 149 MSSA. Samples DNA were extracted and amplified by specific primer of spa gene. In 4 (3.8%) strains of them no spa gene was detected, and 10.6% had a dual band (1200 and 1400 bp). In strains with one band, the length of spa gene differed from 1150 to 1500 bp. The most prevalent length was 1350–1400 bp (37%). The frequencies of short spa bands (1150–1200 bp) in patients strains were significantly higher. In 4 (3.8%) strains of them no spa gene was detected, and 10.6% had a dual band (1200 and 1400 bp). In strains with one band, the length of spa gene differed from 1150 to 1500 bp. The most prevalent length was 1350–1400 bp (37%). The frequencies of short spa bands (1150–1200 bp) in patients strains were significantly higher. The spa gene length of 1350–1400 bp in MSSA was more than in MRSA strains ( ). The average length of spa in isolated strains from urinary tract infections was more than others. It is concluded that the length of spa gene depends either on resistance to Methicillin or the source of S. aureus isolation.